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形狀
lyophilized powder
品質等級
分析物化學類別
proteins (GST)
標籤範圍
≥10 μmol per mL gel
技術
immunoprecipitation (IP): suitable
protein purification: suitable
基質
4% cross-linked beaded agarose
基質活化
epoxy
基質結合
sulfur
基質墊片
12 atoms (10 carbon)
儲存溫度
−20°C
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一般說明
谷胱甘肽硫转移酶(GST)是由天然真核细胞生产的26 KDa分子量蛋白。它属于II相解毒酶家族的一员。谷胱甘肽-琼脂糖树脂由谷胱甘肽通过硫与环氧活化的4%交联琼脂糖珠相连形成12个原子（10碳）的间隔臂。本品为冻干粉末，添加了乳糖稳定剂。
應用
谷胱甘肽琼脂糖树脂用于：
- 纯化谷胱甘肽硫转移酶(GST)融合蛋白，生成多克隆抗体M4P
- 通过孵育上清液组分，纯化全长p53蛋白(aa 1-393)及其DNA结合域(aa 94-288)
- 融合蛋白的亲和纯化
利用谷胱甘肽-琼脂糖亲和层析技术，可以快速、温和、非变性和高度选择性地纯化含有谷胱甘肽结合序列的蛋白质，如谷胱甘肽S-转移酶(GST)、谷胱甘肽过氧化物酶和乙二醛酶I。
生化／生理作用
谷胱甘肽硫转移酶(GST)属于多功能解毒同工酶超家族成员，在细胞信号传导中起重要作用。由于其特定同工酶在多种肿瘤中过表达，并且可能参与神经退行性疾病、多发性硬化症和哮喘等其他疾病的发生发展，GST成为前景可期的治疗靶标。
特點和優勢
- 提供快速、温和、非变性、高度选择性的谷胱甘肽结合酶纯化。
- 可在-20 °C恰当干燥保存2年。
- 载量：每mL树脂可结合5-10 mg谷胱甘肽S-转移酶
數量
1g粉末可溶胀至 ≥10 ml凝胶
外觀
用乳糖稳定化的冻干粉
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
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