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Merck

CS0410

Sigma-Aldrich

谷胱甘肽S-转移酶(GST)活性检测试剂盒

sufficient for 500 multiwell tests

别名:

GST Enzyme Activity Kit

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About This Item

EC號碼:
分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 500 multiwell tests

品質等級

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

一般說明

谷胱甘肽S-转移酶 (GST) 检测试剂盒用于测定总GST的活性。适用于细胞和细菌裂解物、组织匀浆以及血浆
和红细胞裂解物样品。

應用

该谷胱甘肽S-转移酶检测试剂盒采用1-氯-2,4-二硝基苯(CDNB),适用于广泛的GST同工酶。基本原理是谷胱甘肽硫醇基团结合到CDNB底物上后,在340nm处的吸光度会增加。

生化/生理作用

谷胱甘肽S-转移酶 (GST)是在哺乳动物解除许多外源物毒素过程中发挥重要作用的一组酶。该酶可将谷胱甘肽的硫醇基团连接到亲电的外源物上,保护细胞免受毒物损害,进一步帮助细胞抵抗化合物诱变、致癌和毒性作用。GST活性广泛存在于植物、昆虫、酵母、细菌以及大多数哺乳动物组织中,尤其是在解毒过程中发挥关键作用的肝脏中。

仅试剂盒组分

产品编号
说明

  • Dulbecco's Phosphate Buffered Saline 100 mL

  • L-Glutathione Reduced 1 g

  • GST (Control) .1 mL

  • Sample Buffer 5 mL

  • Substrate (CDNB) 1.2 mL

訊號詞

Danger

危險分類

Acute Tox. 2 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Maria Sehlstedt et al.
Particle and fibre toxicology, 7, 21-21 (2010-08-24)
Biomass combustion contributes to the production of ambient particulate matter (PM) in rural environments as well as urban settings, but relatively little is known about the health effects of these emissions. The aim of this study was therefore to characterize
Irvin Bonola-Gallardo et al.
Biological trace element research, 176(1), 40-47 (2016-07-28)
This study was conducted to measure the activity of the enzyme glutathione S-transferase (GST) in saliva and to compare the activity of this enzyme in children with and without dental fluorosis in communities with different concentrations of naturally fluoridated water.
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Environmental toxicology, 32(1), 227-240 (2015-12-18)
Gallic and pelargonic acids are biologically derived substances receiving a growing interest as eco-friendly biocides with potential applications in freshwater system management. However, some data gaps remain to address their chronic ecotoxicity issue, particularly for fish. This work aimed at
T Lin et al.
Toxicology, 235(1-2), 1-10 (2007-04-10)
Glutathione (GSH) is one of the most important antioxidants in mammalian cells. It also plays an important role in chemical detoxification. Some evidence showed that polycyclic aromatic hydrocarbons, such as benzo[a]pyrene (B[a]P [50-32-8]), could increase GSH content as a defense
Daniel Scotcher et al.
Drug metabolism and disposition: the biological fate of chemicals, 45(5), 556-568 (2017-03-09)
In vitro-in vivo extrapolation of drug metabolism data obtained in enriched preparations of subcellular fractions rely on robust estimates of physiologically relevant scaling factors for the prediction of clearance in vivo. The purpose of the current study was to measure

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