推荐产品
包裝
pack of 5 × 1 mL
製造商／商標名
Cytiva 17-5319-01
參數
<20 mL/min flow rate
42 psi
床尺寸
16 mm × 25 mm
床體積
5 mL
柱內徑
16 mm
基質
6% cross-linked agarose
粒徑
45-165 μm
平均直徑
90 μm
cleaning
2-14(Ni2+-stripped medium.)
工作範圍
3-12(Ni2+-stripped medium.)
容量
~40 mg binding capacity(histidine-tagged protein)
適合性
suitable for bioprocess medium
一般說明
Ni Sepharose™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
The high flow rates made possible by the Ni Sepharose™ 6 Fast Flow matrix make HisTrap™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.
特點和優勢
- High binding capacity, approx. 40 mg/mL medium.
- Negligible leakage of Ni2+.
- Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
实验方案
This page shows how to perform a cell disruption und prepare membranes with products from GE Healthcare.
AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.
This page shows how to purify protein A-tagged proteins using GE Healthcare products.
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.
相关内容
A range of purification methods may be used to prepare a sample prior to downstream analysis. Purification reduces sample complexity, removes interferences, and concentrates an analyte before analysis.
Purification methods for downstream analysis reduce sample complexity, remove interferences, and concentrate analytes for more accurate results.
Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.
Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.
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