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Key Documents

GE17-0529-01

Calmodulin-Sepharose 4B

Cytiva 17-0529-01, pack of 10 mL

别名:

Calmodulin Affinity Resin, Calmodulin-Sepharose

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

ligand

calmodulin

包裝

pack of 10 mL

製造商/商標名

Cytiva 17-0529-01

儲存條件

(20% Ehtanol)

基質

4% agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning in place

4-9

工作範圍

4-9

儲存溫度

2-8°C

一般說明

Calmodulin Sepharose 4B affinity media for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells.

Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD?/NADP? Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca2+ on separate Ca2+-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca2+ complexes are particularly stable.

特點和優勢

  • For single-step purification of native calmodulin-binding proteins
  • Suitable for tandem affinity purification (TAP) of protein complexes
  • Purification of calmodulin-regulated proteins from all eukaryotic cells

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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Mahmoud Hajj Chehade et al.
Cell chemical biology, 26(4), 482-492 (2019-01-29)
Ubiquinone (UQ) is a polyprenylated lipid that is conserved from bacteria to humans and is crucial to cellular respiration. How the cell orchestrates the efficient synthesis of UQ, which involves the modification of extremely hydrophobic substrates by multiple sequential enzymes

商品

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from GE Healthcare.

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

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相关内容

Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

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