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Key Documents

GE17-0032-01

Sigma-Aldrich

Sephadex® G-25 Fine

Cytiva 17-0032-01, pack of 100 g

别名:

Sephadex G-25 Medium

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About This Item

分類程式碼代碼:
23151817
NACRES:
NA.56

包裝

pack of 100 g

製造商／商標名

Cytiva 17-0032-01

儲存條件

dry media

基質

cross-linked dextran

粒徑

>20 μm (dry)

cleaning in place

2-13

工作範圍

2-13

適合性

suitable for bioprocess medium

相关类别

一般說明

Sephadex® G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-25 is available in 4 different particle sizes (Course, Medium, Fine & Superfine).

Sephadex® G-25 is available in different bulk pack sizes and convenient pre-packed formats.
As a BioProcess medium, Sephadex® G-25, meets industrial demands with security of supply and comprehensive technical and regulatory support.

特點和優勢

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Excellent recovery and minimum sample dilution
  • Available in prepacked HiPrep™ Desalting columns for fast and convenient desalting
  • BioProcess medium supported for industrial applications and well-established for desalting and buffer exchange in industrial applications

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

取代透過

产品编号
说明
价格

G2580

Sephadex® G-25, Fine

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

Fractional Precipitation Protocol for Affinity Chromatography Samples

This page shows describes fractional precipitation for removing gross impurities from affinity chromatography samples with small volumes.

Performing a Separation with Sephadex®

This page shows how to perform a separation with a Sephadex column from GE Healthcare which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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