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Merck

G0799

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于大肠杆菌

>20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution

别名:

β-D-葡萄糖苷酸葡萄糖醛酸水解酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

重組細胞

expressed in E. coli

品質等級

形狀

aqueous glycerol solution

比活性

>20,000,000 units/g protein

分子量

74 kDa

濃度

>1 mg/mL

運輸包裝

wet ice

儲存溫度

−20°C

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相关类别

一般說明

β-葡萄糖醛酸酶是属于糖苷酶家族的四聚体,分子量为 29 kDa。它由 74 kDa 相同的单体组成。该酶的 pI 和最适 pH 值分别为 4.8 和 6-7。

應用

β-葡萄糖醛酸酶用于研究Cer-β葡萄糖苷酸的合成和评估来自大肠杆菌或肠段的释放头部基团的β葡萄糖醛酸酶。
比较不同酶性能的新技术文章
了解更多
了解Sigma研发获得的最新应用数据,关于使用不同来源的酶优化不同药物类别的水解,并使用色谱纯化酶降低酯酶活性导致6-单乙酰吗啡(6-MAM)转化为吗啡的影响
有效水解类固醇葡糖苷酸。

單位定義

一个Sigma或改良的Fishman单位将在37℃,pH6.8(30分钟测定)下每小时从酚酞葡糖苷酸中释放1.0μg酚酞。

外觀

β葡萄糖醛酸酶在GUS测定中可用作报告基因以监测基因的表达。
含50%甘油的高纯度溶液

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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D H Kim et al.
Biological & pharmaceutical bulletin, 18(9), 1184-1188 (1995-09-01)
beta-Glucuronidase was purified 360-fold from Escherichia coli HGU-3, an human intestinal bacterium. The specific activity of the purified enzyme was 17.78 units/mg protein. The enzyme (M.W. 290000) is composed of four subunits (M.W. 72000) with a pI and optimal pH
E M Schmelz et al.
Cancer research, 59(22), 5768-5772 (1999-12-03)
Dietary sphingolipids inhibit chemically induced colon cancer in mice. The most likely mediators of this effect are the metabolites ceramide (Cer) and sphingosine, which induce growth arrest and apoptosis in transformed cells. Sphingolipids are digested in both the upper and
Xiaoyan Liu et al.
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 42(1), 67-74 (2013-03-19)
To construct a lentiviral RNA interference system targeting heparanase (HPSE) based on miR30 and to test its silencing effect. Three heparanase-shRNA structures were designed based miR30. The targeting fragments were obtained by PCR, then inserted into the vector LV PP-GFP
G Cassinelli et al.
Biochemical pharmacology, 85(10), 1424-1432 (2013-03-08)
The activity of heparanase is responsible for heparan sulfate cleavage, thus resulting in the release of heparan sulfate-bound growth factors. Since heparanase activity is upregulated in several tumor types and is implicated in the malignant behavior, the enzyme is regarded
Fei Song et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 67(11-12), 611-619 (2013-02-19)
Genes coding for avenin-like proteins (ALP) represent a new family of wheat storage protein genes. To find a wheat endosperm-specific promoter, a 1644-bp fragment upstream of the ALP type-B gene (GenBank accession number JN622144) was isolated. The important promoter elements

实验方案

To optimize hydrolysis using β-glucuronidase, factors such as incubation time, temperature, hydrolysis pH, enzyme source, and enzyme concentration must be evaluated for each glucuronide metabolite to be analyzed.

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