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一般說明
来自罗马蜗牛中的H-2型β-葡萄糖醛酸酶是一种来源于罗马蜗牛的酶粗溶液。许多毒素软体动物的β-葡糖醛酸酶也含有硫酸酯酶活性。
應用
β-葡糖苷酸酶来自于罗马蜗牛，可用于
- 在肝素化血浆样品中水解葡糖醛酸结合的代谢物
- 用于测定1'-羟基咪达唑仑（1'-OHMDZ）的血浆样品预处理中
- 用于处理虹鳟鱼的胆汁样品，用于水解葡萄糖醛酸和硫酸盐代谢产物
- 用于处理来自产孢培养基的细胞，以完全消化子囊壁
采用β-葡萄糖醛酸腺苷酸酶分解的组织匀浆，进行液相色谱-电喷雾离子计数质谱（LC/MSn）分析，研究黄芩苷降解产物的结构。在GUS分析中用作报告基因，以监控基因表达。
品質
来自软体动物的多种β-葡糖醛酸糖苷酶也含有硫酸酯酶活性。
單位定義
在pH 5.0、温度37°C条件下，一单元sigma或改良的Fishman单元1小时会从酚酞葡糖苷酸释放 1.0 μg酚酞（30分钟实验）。
硫酸酯酶单元定义：一单位的硫酸酯酶每小时，pH5.0，37℃能够水解1.0 μmole p-硝基儿茶酚硫酸盐。
外觀
在~1.0 M硫酸铵中含有3 mM叠氮化钠作为保存剂的水相溶液。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
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商品
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
实验方案
Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver
Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver. This procedure applies to all b-Glucuronidase products that are derived from Helix Pomatia and Bovine Liver. It is the responsibility of trained Analytical Services laboratory personnel to follow this procedure as written.
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