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Merck
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主要文件

D9281

Sigma-Aldrich

Lambda DNA EcoR I Hind III Digest

for DNA electrophoresis

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About This Item

分類程式碼代碼:
41141827
NACRES:
NA.25

品質等級

100
200

形狀

liquid

用途

100 uses (0.1 mg size)
500 uses (0.5 mg size)

適合性

suitable for electrophoresis (DNA)

儲存溫度

−20°C

一般說明

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

應用

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

成分

10mM Tris-HCl 中的溶液,pH 8.0,1mM EDTA

其他說明

Contains 13 fragments, 125–21,226 bp
For optimal resolution, the recommended agarose gel concentration is 0.7%.

準備報告

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Skin Sens. 1

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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商品

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

实验方案

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

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