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Merck

D9655

Sigma-Aldrich

pBR322 Hae III Digest

for DNA electrophoresis

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.25

品質等級

形狀

liquid

用途

1000 uses (0.5 mg size)
200 uses (0.1 mg size)

適合性

suitable for electrophoresis (DNA)

儲存溫度

−20°C

一般說明

Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

應用

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

成分

10mM Tris-HCl 中的溶液,pH 8.0,1mM EDTA

其他說明

Contains 22 fragments, 8–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5.

準備報告

Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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商品

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

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