跳转至内容
Merck

D0690

Sigma-Aldrich

DNA Gyrase 来源于大肠杆菌

aqueous glycerol solution

登录查看公司和协议定价


About This Item

MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Escherichia coli

品質等級

形狀

aqueous glycerol solution

分子量

~374 kDa

濃度

≥2 unit/μL

技術

cell based assay: suitable

UniProt登錄號

應用

cell analysis

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

Escherichia coli K12 ... gyrA(946614) , gyrB(948211)

應用

来自 大肠杆菌 的 DNA 旋转酶已用于研究比较蛋白质组学方法,以更好地定义拟核异常球菌的特异性。来自 大肠杆菌 的 DNA 旋转酶也用于研究DnaK-ClpB bichaperone系统在 DNA 旋转酶再激活中的作用。

生化/生理作用

DNA 旋转酶以A 2 B 2 全酶的形式提供。亚单位 A 的分子量为 97 kDa,亚单位 B 的分子量为 90 kDa。它催化负性超螺旋 ATP 依赖性引入松弛 DNA 这一过程。通过诱变实验,已成功地将 DNA 旋转酶转化为 II 类型拓扑异构酶。
可用于超螺旋质粒。

單位定義

在 37°C下,一个单位的旋转酶活性将在30分钟内超螺旋 0.5 微克的 pBR-322 DNA。

其他說明

溶于含 Tris 缓冲液、DTT 和 EDTA 的 50% 甘油中。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

S C Kampranis et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(25), 14416-14421 (1996-12-10)
DNA gyrase is unique among topoisomerases in its ability to introduce negative supercoils into closed-circular DNA. We have demonstrated that deletion of the C-terminal DNA-binding domain of the A subunit of gyrase gives rise to an enzyme that cannot supercoil
Alix Pantel et al.
Antimicrobial agents and chemotherapy, 56(4), 1990-1996 (2012-02-01)
Fluoroquinolone (FQ) resistance is emerging in Mycobacterium tuberculosis. The main mechanism of FQ resistance is amino acid substitution within the quinolone resistance-determining region (QRDR) of the GyrA subunit of DNA gyrase, the sole FQ target in M. tuberculosis. However, substitutions
Magali Toueille et al.
Journal of proteomics, 75(9), 2588-2600 (2012-03-27)
Compared to radiation-sensitive bacteria, the nucleoids of radiation-resistant Deinococcus species show a higher degree of compaction. Such a condensed nucleoid may contribute to the extreme radiation resistance of Deinococcus by limiting dispersion of radiation-induced DNA fragments. Architectural proteins may play
Teresa Lara-Ortíz et al.
Canadian journal of microbiology, 58(2), 195-199 (2012-01-24)
In Escherichia coli cells, an increase in temperature induces immediate DNA relaxation, followed by the fast recovery of DNA supercoiling. DNA gyrase, proteins synthesized during heat stress, and chaperone DnaK have been proposed to participate in this recovery. However, the
Valentina Monica et al.
Lung cancer (Amsterdam, Netherlands), 79(3), 228-235 (2013-01-02)
Thymic epithelial tumors include several entities with different biologic behavior. Chemotherapy is indicated in advanced disease, but limited data exist on gene expression correlation with the response to chemotherapeutic agents. A series of 69 thymic neoplasms (7 A-, 6 AB-

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门