推荐产品
生物源
microbial
化驗
≥90%
形狀
liquid
濃度
100 mg/mL in water
顏色
faint yellow to dark brown
抗生素活性譜
Gram-negative bacteria
Gram-positive bacteria
mycobacteria
neoplastics
作用方式
DNA synthesis | interferes
enzyme | inhibits
儲存溫度
−20°C
一般說明
Novobiocin, also known as albamycin or cathomycin, is an aminocoumarin antibiotic commonly derived from Streptomyces Sp. It exerts its antibacterial activity by inhibiting Topo II α, which corresponds to bacterial DNA gyrase. It is effective against both gram-positive and gram-negative bacteria and is significant in research involving highly beta-lactam-resistant pneumococcal infections and anti-orthopoxvirus applications. Functioning as a competitive inhibitor in the ATPase reaction catalyzed by GyrB, Novobiocin is employed in microbiology and cell biology research to effectively prevent the growth of bacterial contaminants.
應用
Novobiocin has been used:
- in cell culture systems to treat mycoplasma contaminations.[3]
- in a research to where it has exhibited anti-cancer effects by inhibition of Hsp90 (heat shock protein)[4],[5]
生化／生理作用
Mode of Action: Inhibits DNA synthesis by inhibiting the enzyme Topoisomerase II
Activity spectrum: Active against gram-positive and gram-negative bacteria
Activity spectrum: Active against gram-positive and gram-negative bacteria
特點和優勢
- Ready available solution reduce the need for preparation time
- Commonly used in Cell Biology and Biochemical applications
- High quality antibiotic suitable for mulitple research applications
準備報告
Novobiocin Ready Made Solution is light sensitive. Thaw this product at room temperature. It is recommended to avoid repeated freeze thaw cycles of this product. The suggested concentration of Novobiocin for cell culture assays is 50-1000 μg/mL,,, therefore, it is recommended to dilute the Ready Made Solution 1:100-2000.
其他說明
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訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
Environmentally constrained mutation and adaptive evolution in Salmonella.
Massey, R. C., Rainey, P. B., Sheehan, B. J., Keane, O. M., & Dorman, C. J.
Current Biology, 9, 1477-1481 (1999)
Biosynthesis of novobiocin by Streptomyces niveus.
Kominek, L. A.
Antimicrobial Agents and Chemotherapy, 1, 123-134 (1972)
Synthesis and biological evaluation of arylated novobiocin analogs as Hsp90 inhibitors.
Kusuma, B. R., Duerfeldt, A. S., & Blagg, B. S.
Bioorganic & Medicinal Chemistry Letters, 21, 7170-7174 (2011)
Mycoplasmas in tissue culture.
Macpherson, I.
Journal of Cell Science, 1, 145-168 (1966)
Novobiocin antagonism of amastigotes of Trypanosoma cruzi growing in cell-free medium.
Pate, P. G., Wolfson, J. S., McHugh, G. L., Pan, S. C., & Swartz, M. N.
Antimicrobial Agents and Chemotherapy, 29, 426-431 (1986)
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