NCI-H322 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95111734, human lung (cervical node metastasis), Not specified

• 别名: H322 Cells, NCI-322 Cells, NCI-H322T Cells
• 分類程式碼代碼: 41106514

属性

• 生物源: human lung (cervical node metastasis)
• 描述: Human Caucasian bronchioalveolar carcinoma
• 包裝: tube of 5 μg 95111734-DNA-5UG; pkg of vial of cells 95111734-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian bronchioalveolar carcinoma

細胞系描述

NCI-H322 was derived in 1981 from a primary bronchioalveolar carcinoma of the lung from a 52 year old male taken prior to treatment. Ultrastructural studies of this non-small cell carcinoma cell line demonstrated the presence of cytoplasmic structures characteristic of Clara cells. NCI-H322 do not express UDP-glucuronosyltransferase, but are reported to express glutathione-S-transferase and phenol sulfotransferase. Expression of protein and RNA of SP-A, the major surfactant-associated protein was detected. The cells produce tumours in athymic mice.

應用

NCI-H322 has been used to study the preclinical antitumor efficacy of BAY 1129980, which is an anti-C4.4A (Ly6/PLAUR domain-containing protein 3 precursor-LYPD3) antibody–drug conjugate for the treatment of non–small cell lung cancer.

Test system for evaluating cytotoxicity and genotoxicity of chemicals to human lung

培養基

RPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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