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Key Documents

DMS 273

95062830, human lung (effusion), Not specified

别名:

DMS273 Cells

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About This Item

分類程式碼代碼:
41106514

product name

DMS 273, 95062830

生物源

human lung (effusion)

增長模式

Adherent

染色體組型

Not specified

形態學

Not specified

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Human lung, small cell carcinoma

細胞系描述

DMS 273 was initiated in 1978 from the pleural fluid specimen from a 50 year old female patient with small cell carcinoma of the lung. The patient was in relapse after chemotherapy and radiotherapy. DMS 273 cells exhibit intercellular processes and express retinoblastoma mRNA and protein. The cell line is tumorigenic in nude mice.

應用

Human lung tumour model

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10 ,12
D13S317: 12,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 10
vWA: 15

培養基

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

其他說明

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