LC-2/ad

94072247, human lung, Epithelial

• 别名: LC-2/ad Cells
• 分類程式碼代碼: 41106514

属性

• product name: LC-2/ad, 94072247
• 生物源: human lung
• 增長模式: Adherent
• 染色體組型: Modal No 53-56
• 形態學: Epithelial
• 產品: alpha-1-anti-trypsin
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human lung adenocarcinoma

細胞系描述

Established from the pleural effusion of a pulmonary adenocarcinoma in a 51 year-old Japanese female. LC-2/ad cells exhibit an epithelial appearance and a tendency to form small domes. Immunocytochemically the cells are positive for CEA and cytokeratins, including cytokeratin No.18. LC-2/ad produces at least two functionally active trypsin inhibitors together with several proteinases in vitro.

應用

LC-2/ad has been used to study the ability of RXDX-105 as a therapy for cancers which are affected by RET (proto-oncogene tyrosine-protein kinase receptor) changes.

培養基

RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Split confluent cultures 1:2-1:4 ie seeding at 2-5 x 10,000 cells/cm² using 0.25% trypsin or trypsin/ EDTA ; 5%CO₂; 37°C

其他說明

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