DMS 153

95062827, human lung (liver metastasis), Not specified

• 分類程式碼代碼: 41106514

属性

• product name: DMS 153, 95062827
• 生物源: human lung (liver metastasis)
• 增長模式: Semi-adherent aggregates
• 染色體組型: Modal no. 41-42
• 形態學: Not specified
• 產品: Not specified
• 受體: Bombesin, Epidermal growth factor (EGF)
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian lung small cell carcinoma, metastasis to liver

細胞系描述

DMS 153 was isolated from the liver of a 44 year-old male patient at autopsy who had been treated with cytoxan and methotrexate. The cells were identified as small cell carcinoma of the lung (SCLC) and secrete moderate quantities of calcitonin (CT) and calcitonin gene related peptide (CGRP). Production of adrenocorticotropin (ACTH), bombesin (BN), 17 β-estradiol and oxytocin-neurophysin (OT-NP) has also been reported. The expression of cell surface antigens Leu-7, HLA-DR, My 23 (defined by AML-2-23 monoclonal antibody), the AML-1-99 defined antigen and CR3 receptor (defined by the OKM antibody) has been shown. DMS 153 cells produce receptors for BN and epidermal growth factor (EGF) and are tumourigenic in athymic, nude mice.

應用

Human lung tumour model

DNA分析

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10
D13S317: 13
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 7,9.3
TPOX: 11
vWA: 14,15

培養基

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.Cells grow in large clumps.

其他說明

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