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Merck
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Key Documents

NP cl.25

Reactivity: Anti-Measles Virus Nucleoprotein, 95051114

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About This Item

分類程式碼代碼:
41106514

生物源

Mouse x Mouse Hybridoma

ImmunoDon

BALB/c mouse spleen

骨髓瘤

Sp2/0 Ag-14

反應

Anti-Measles Virus Nucleoprotein

技術

cell culture | mammalian: suitable

同型

IgG2a

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Mouse Sp2/0 x Mouse BALB/c spleen

細胞系描述

NP cl.25 monoclonal antibody was produced in vitro by fusing mouse myeloma cells with spleen cells derived from BALB/c mice immunised with purified measles virus. NP cl.25 is negative for haemagglutination and hemolysin inhibition as well as neutralisation assays and gives immunofluorescence only on fixed tissue. It has been used for measuring synthesis and topological accumulation of measles virus antigen during the lytic cycle.

免疫原

Measles virus (strain Hallè)

應用

Diagnosis, study of measles virus infection

培養基

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Maintain cultures between 1-5x100,000 cells/ml; 5% CO2; 37°C

其他說明

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