MDBK Cell Line bovine

90050801, bovine kidney, Epithelial-like

• 别名: MDBK (NBL-1) Cells, Madin Darby Bovine Kidney Cells, NBL-1 Cells
• 分類程式碼代碼: 41106514
• eCl@ss: 32190102

属性

• product name: MDBK Cell Line bovine, 90050801
• 生物源: bovine kidney
• 增長模式: Adherent
• 染色體組型: Modal no. 51; hypodiploid
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Bovine kidney

細胞系描述

Derived from the kidney of an apparently normal, adult Bos taurus. Also known as MDBK (NBL-1).

應用

MDBK cell line has been used to study the expression of Eimeria bovis-derived antigens on the host cell surface membrane in vitro. It has also been used to study the ubiquitination and degradation of signal transducer and activator of transcription 1 and 2 (STAT1 and STAT2) by the V proteins of the paramyxoviruses simian virus 5 and human parainfluenza virus type 2.

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or 10% Horse Serum (HS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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