BF-11

Reactivity: Anti-Erythropoietin, human, 95050413

• 分類程式碼代碼: 41106514

属性

• 生物源: Rat x Mouse Hybridoma
• ImmunoDon: Rat AC1 spleen cells
• 骨髓瘤: Sp2/0 Ag-14
• 反應: Anti-Erythropoietin, human
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat ACI spleen x Mouse Sp2/0 Ag-14

細胞系描述

The BF-11 hybridoma cell line secretes a rat monoclonal antibody named Epoclonalan that reacts with human erythropoietin. Spleen cells from ACI strain rats that had been immunised with human urinary erythropoietin have been fused with Sp2/0 Ag-14 to produce this cell line. The α, β and asialo forms of human erythropoietin are bound by the antibody to the same extent.

免疫原

Human erythropoietin

應用

Immunological studies, isolation of erythropoietin by immuno-affinity methods.

The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

培養基

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO₂; 37°C.

其他說明

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