SW 403

87071008, human colon, Epithelial

• 分類程式碼代碼: 41106514

属性

• 产品名称: SW 403, 87071008
• 生物源: human colon
• 增長模式: Adherent
• 染色體組型: 2n = 46, triploid
• 形態學: Epithelial
• 產品: Carcinoembryonic antigen (CEA)
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian colon adenocarcinoma

細胞系描述

Established from a grade III adenocarcinoma of the bowel of a 51 year old Caucasian female. The cells produce CEA and are tumorigenic in nude mice. Electron microscopy reveals prominent golgi apparatus.

應用

Tumourigenicity studies

培養基

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin/EDTA; No CO₂; 37°C. Cells do not reach confluency but will grow in small islands.

其他說明

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