SW 1417

90102543

• 分類程式碼代碼: 41106514

属性

• 生物源: human colon
• 增長模式: Adherent
• 染色體組型: 2n = 46
• 形態學: Epithelial
• 產品: Carcinoembryonic antigen (CEA)
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human colon adenocarcinoma

細胞系描述

Derived from a grade III adenocarcinoma of the colon in a 53 year old female. CEA is produced and electron microscopy reveals the presence of brush borders around colonies in vitro.

應用

Tumourigenicity studies

培養基

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; No CO₂; 37°C. Centrifugation and resuspension are recommended to disperse clumps.

其他說明

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