RPMI 8226

87012702

• 别名: RPMI8226 Cells, Roswell Park Memorial Institute 8226 Cells
• 分類程式碼代碼: 41106514

属性

• 生物源: human blood
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Immunoglobulin G (Ig) lambda light chain
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human myeloma

細胞系描述

Derived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.

應用

RPMI 8226 has been used:

• to grow human myeloma cell lines
• to evaluate the effect of 1-aryl-2-methylthioimidazolines (IIV) on the viability of RPMI 8226 cells
• to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. After resuscitation cell debris will be present in culture. This will decrease with subsequent subculture.

其他說明

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