M3817

MegaCell^™ RPMI-1640 Medium

without L-glutamine, liquid, sterile-filtered, suitable for cell culture

• 分類程式碼代碼: 12352207
• NACRES: NA.75

属性

• 品質等級: 500
• 無菌: sterile-filtered
• 形狀: liquid
• 技術: cell culture | mammalian: suitable
• 雜質: endotoxin, tested
• 成分: L-glutamine: no
• 運輸包裝: ambient
• 儲存溫度: 2-8°C

说明

一般說明

MegaCell^™ is a line of versatile media, formulated to significantly reduce the amount of serum required for cultivating mammalian cells in vitro. It is effective as a basal formulation for the growth and maintenance of adherent or suspension cell lines. When supplemented with 3% fetal bovine serum (FBS), MegaCell supports proliferative rates and maximal cell densities comparable, and in some cases superior, to the conventional basal formulation supplemented with 10% fetal bovine serum.

應用

MegaCell^™ RPMI-1640 Medium has been used:

• as a component of the TSC medium for culturing trophoblast stem cells (TSC)
• as a component of 25 ng/ml FGF4 and 1 g/ml heparin (TSF4H medium) for culturing TSCs
• as a component of embryo-like structures (ETS) embryo medium and TS cell medium for culturing embryonic stem cells (ESCs) and TSCs

重構

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

法律資訊

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.

MegaCell is a trademark of Promega Corp.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable