推荐产品
product name
RPMI-1640 培养基, Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture
形狀
powder
技術
cell culture | mammalian: suitable
成分
HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
應用
RPMI-1640培养基被用于以下应用：
- 用于细胞培养基：补充其他组分的RPMI-1640培养基可用于培养鼠胸腺瘤EL-4细胞
- 不含抗生素的RPMI 1640培养基可用于脾细胞的分枝杆菌感染
- 用于细胞分离和培养
- 作为培养基组分之一，用于淋巴细胞分离和培养、ELISpot酶联免疫斑点测定、离体增殖试验
- 作为培养基，用于gp39基因培养和CD40免疫球蛋白结合试验
- 抗真菌剂制备
數量
按照每升培养基10.4克粉末配制。
重構
补充2.0 g/L碳酸氢钠。
其他說明
酚红会干扰一些细胞在克隆密度下的生长。该培养基适用于干细胞培养或低密度的细胞培养。也推荐用于体外诊断。
也與該產品經常一起購買
产品编号
说明
价格
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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商品
The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.
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