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product name
StableCell™ DMEM - 高葡萄糖, With 4500 mg/L glucose, stable glutamine, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
無菌
sterile-filtered
形狀
liquid
技術
cell culture | mammalian: suitable
雜質
Endotoxin, tested
成分
glucose: high
HEPES: no
stable glutamine
sodium pyruvate: yes
phenol red: yes
NaHCO3: yes
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
该DMEM-Hi葡萄糖培养基与原始配方不同，因为它补充有L-丙氨酰-L-谷氨酰胺二肽。 众所周知，谷氨酰胺在细胞培养中不稳定。该二肽提供更稳定形式的谷氨酰胺用于细胞培养。
應用
Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。
法律資訊
StableCell is a trademark of Sigma-Aldrich Co. LLC
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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The EMBO journal, 30(21), 4479-4488 (2011-09-29)
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