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产品名称
杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture
品質等級
無菌
sterile-filtered
形狀
liquid
技術
cell culture | mammalian: suitable
雜質
endotoxin, tested
成分
glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
在成分确定的培养基研究中，通常用成分确定的混合物（包括营养素、生长因子和激素）替代血清添加到培养基中。常用的组合是Dulbecco′s改良Eagle培养基(DME)与Ham′s F-12营养混合物（1:1混合）。为了补偿去血清后的缓冲能力损失，可在配方中添加15 mM终浓度的HEPES缓冲液，帮助研究人员优化缓冲体系。
應用
Dulbecco′s改良Eagle培养基/Ham F-12营养混合物已用于：
- 维持人肺和结直肠癌细胞系生存，进行次级代谢产物的生物学评估
- 培养人肾-2细胞(HK-2)并作为检测培养基组分，用于溶血磷脂酰胆碱(LPC)处理
- 小鼠鳞状细胞癌(SCCVII)肿瘤细胞培养
也與該產品經常一起購買
产品编号
说明
价格
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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