AB12 cell line from mouse lung

10092306

• 分類程式碼代碼: 41106514

属性

• 生物源: mouse lung
• 描述: Malignant mesothelioma from mouse lung
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Cells are stellate-shaped
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse malignant mesothelioma

細胞系描述

Mice (BALB/c female 6-8 weeks) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0146

應用

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

培養基

RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 5% FCS

例行更新培養

Optimal split ratio 1:16 - 1:32 (seeding density 0.8 x104 cells/cm²). Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake - University of Western Australia, Australia