CAD cell line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 8100805, mouse nerve, Neuronal

• 别名: CAD Cell Line
• 分類程式碼代碼: 41106514

属性

• 生物源: mouse nerve
• 描述: Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumor
• 包裝: tube of 5 μg 08100805-DNA-5UG; pkg of vial of cells 08100805-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Neuronal
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumour

細胞系描述

CAD cells (Cath.-a-differentiated) are a variant of a CNS catecholaminergic cell line established from a brain tumour in a transgenic mouse carrying wild-type SV40 T antigen under transcriptional control of rat tyrosine hydroxylase promoter. CAD has lost the immortalising oncogene and expresses neurone-specific proteins and synaptic vesicle proteins. CAD will differentiate to form neuronal processes in serum free conditions.

應用

Study of neuronal differentiation

培養基

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 8% Foetal Bovine Serum (FBS)

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 X 10⁴ cells/cm² Cells should be dislodged by gentle pipetting; 5% CO₂; 37°C.

其他說明

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