N18TG2

8062523, mouse nerve, Neuronal

• 分類程式碼代碼: 41106514

属性

• product name: N18TG2, 08062523
• 生物源: mouse nerve
• 增長模式: Adherent
• 染色體組型: mean chromosome number is 89
• 形態學: Neuronal
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse neuroblastoma

細胞系描述

A 6-thioguanine-resistant clone of N18 (a clonal line produced from the mouse neuroblastoma C1300). (Mouse strain A/Jax). This cell line exhibits high levels of acetylcholinesterase. Found to be tumourigenic in BALB/c nude mice.

應用

Used in the generation of cell hybrids.

培養基

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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