CAS9PL

Cas9 Plus Protein

from Streptococcus pyogenes, recombinant, expressed in E. coli, 3X NLS

• 别名: Cas9, SpCas9, SpyCas9
• 分類程式碼代碼: 12352200
• NACRES: NA.51

属性

• 重組細胞: expressed in E. coli
• 品質等級: 200
• 化驗: ≥95% (SDS-PAGE)
• 形狀: lyophilized powder
• 包裝: pkg of 1 kit (4 components)
• 應用: CRISPR
• 運輸包裝: wet ice
• 儲存溫度: −20°C

说明

一般說明

Recombinant Cas9 Plus protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, Streptococcus pyogenes Cas9 Plus protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.

應用

Functional Genomics; Target Validation; Genome Editing

特點和優勢

• Enhanced specificity compared to wild type Cas9
• Increased activity compared to enhanced specificity Cas9
• Ready to inject/transfect

包裝

pkg of 50 μg (≥ 300 pmol)
pkg of 250 μg (≥ 1500 pmol)

成分

Each kit consists of:

• one vial of Cas9 Plus Recombinant Protein
• one vial of Plus Transfection Buffer
• one vial of Reconstitution Buffer
• one vial of Dilution Buffer

原則

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

重構

Lyophilized S. pyogenes Cas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.

其他說明

Use our CRISPR Selection Tool to order gRNA

Check out our other MISSION^® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins

法律資訊

CRISPR Use License Agreement

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 10 - Combustible liquids