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生物源
Porcine pancreas
品質等級
等級
Proteomics Grade
形狀
lyophilized powder
比活性
≥125 units/mg protein
分子量
34,000 Da± 600
純化經由
affinity chromatography
成份
Protein, 40-70%
異物活動
carboxypeptidase A ≤1%
chymotrypsin ≤0.1%
trypsin ≤5%
儲存溫度
−20°C
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
InChI 密鑰
TWURVFFNODFJBJ-UHFFFAOYSA-N
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相关类别
應用
来自Sigma的酶已被用于开发均相时间分辨荧光（HTRF）检测法，以用于以小型化的高通量筛选形式测定羧肽酶B活性。它已被用于评估C-末端赖氨酸在人纤溶酶原结合至双歧杆菌过程中的影响。使用流式细胞术对用羧肽酶B（一种C-末端赖氨酸特异性内肽酶）处理的效果进行分析和检测。
羧肽酶B已被用于开发用于圈养和野生北极熊的非侵入性妊娠试验。羧肽酶B已被用于确定急性胰腺炎的新潜在生物标志物。
生化／生理作用
羧肽酶B是一种蛋白水解酶，能够快速水解肽键，从而从肽和蛋白质中释放某些羧基末端碱性氨基酸。其分子量为34,300±600 Da。其每摩尔含有一克不可透析的锌原子。金属螯合剂1,10-菲咯啉、8-羟基喹啉-5-磺酸、和2,2′-联吡啶可抑制该酶活性。
包裝
基于蛋白含量进行包装
單位定義
在pH7.65，25℃条件下，一个单位每分钟可水解1.0 μmole马尿酰-L-精氨酸。
外觀
含有HEPES缓冲盐、氯化锌和碳水化合物
準備報告
用蛋白酶抑制剂AEBSF进行处理，以消除丝氨酸蛋白酶活性。
分析報告
蛋白质由缩二脲进行确定。
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
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Laboratory diagnosis of acute pancreatitis: in search of the Holy Grail.
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