推荐产品
生物源
human pancreas
品質等級
形狀
solution
比活性
50-55 units/mg protein carboxypeptidase B
雜質
≤0.2% chymotrypsin
≤0.2% trypsin
≤1 unit/mg protein carboxypeptidase A
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
InChI 密鑰
TWURVFFNODFJBJ-UHFFFAOYSA-N
基因資訊
human ... CPB1(1360)
相关类别
一般說明
羧肽酶B定位于人类染色体3q24上。 羧肽酶B属于羧肽酶的A/B亚家族。
應用
来自Sigma的羧肽酶B作为参考标准品,已被用作测定源自大鼠前叶和中叶的裂解垂体颗粒中羧肽酶活性。 该酶还已被用于通过去除C末端碱性氨基酸对血浆样品进行消化,从而在C4电泳过程中为每种同种异型片段获得独特的条带。
生化/生理作用
羧肽酶B(CPB1)基因的突变与胰腺癌发生和发展的敏感性增加有关。 CPB1水平升高与低度乳腺肿瘤和淋巴结阳性1级肿瘤相关。
羧肽酶B(或肽基-L-赖氨酸(-L-精氨酸)水解酶)可催化碱性氨基酸,赖氨酸、精氨酸和鸟氨酸从多肽的C端水解。研究显示其为34,000Da的单链多肽。胰蛋白酶能够在体外将天然酶转化为活性酶羧肽酶B II。 其最适pH为9.0。该酶可通过连续切割C端碱性氨基酸从而用于序列分析。它也可以用作诊断急性胰腺炎的血清标志物。
單位定義
在pH7.7,25℃条件下,一个单位每分钟可水解1 μmole马尿酰-L-精氨酸
外觀
溶于0.05 M NaOAc(pH 5.0)、1.0 M NaCl、0.01%NaN3中的溶液
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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