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product name
胶原蛋白 来源于人类胎盘, Bornstein and Traub Type IV, powder
生物源
human placenta
品質等級
描述
Sigma Type VI
形狀
powder
雜質
HIV, hepatitis B and hepatitis C, none detected
溶解度
0.5 M acetic acid: 1 mg/mL
適合性
suitable for substrate for collagenase
應用
diagnostic assay manufacturing
儲存溫度
−20°C
基因資訊
human ... COL4A2(1284) , COL4A3(1285) , COL4A4(1286) , COL4A5(1287) , COL4A6(1288)
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生化/生理作用
在发育过程中,胶原蛋白IV普遍分布在BM中。 在成熟过程中,该网状纤维以显著的组织特异性方式被部分替换,从而定义BM结构和功能。 胶原蛋白IV可以结合血小板、肝细胞、角质形成细胞、内皮细胞、肾小球膜细胞、胰腺细胞以及一些肿瘤细胞。
成分
所有胶原蛋白分子均由以三螺旋构象排列的三条肽链组成,其一级结构主要以三个氨基酸作为重复基序,即每隔两个氨基酸是一个甘氨酸残基,其前面的两个位置通常为脯氨酸或4-羟基脯氨酸。 IV型胶原仅存在于基底膜中,并含有多达六条基因不同的a链。
準備報告
本品为冻干粉末,能够以1 mg/mL的浓度重悬于无菌的.5 M乙酸、PBS或水中。 PBS能够在-20℃情况下稳定保存至少一年。它是通过改良版 Glanville, R.W., et al胃蛋白酶提取蛋白制备的。
分析報告
SDS聚丙烯酰胺凝胶电泳在和基底膜胶原蛋白相同的还原条件下测试,得到三条主要条带。
其他說明
胶原蛋白被分为许多种结构和遗传性不同的类型。 我们使用 Bornstein和Traub提出的系统命名法。 小心不要混淆Sigma命名系统和通用的胶原蛋白分类类型。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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