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Merck
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文件

CC076

Sigma-Aldrich

人IV型胶原蛋白

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.75

生物源

human

品質等級

化驗

95% (Human collagen type IV, SDS-PAGE)

形狀

liquid

分子量

300 kDa

製造商/商標名

Chemicon®

濃度

1 mg/mL

技術

cell culture | mammalian: suitable

雜質

<5% Human collagen types I-III, V, VI, and non-collagen proteins

輸入

sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

溶解度

water: soluble at 20 °C

NCBI登錄號

UniProt登錄號

結合特異性

Peptide Source: Laminin

Peptide Source: Nidogen

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

human ... COL4A1(1282)

相关类别

一般說明

IV型胶原是基底膜的主要结构元素。 IV型人胶原蛋白是天然的三螺旋。 轻度胃蛋白酶治疗后,将胶原蛋白从洗涤过的解剖组织中提取到稀乙酸中。 通过使用差异盐沉淀纯化IV型胶原。 天然螺旋结构的纯度和保留通过SDS-PAGE和与抗胶原类型特异性单克隆抗体的反应来控制。 分子量:300kDa;溶解度:10mg/mL
产品来源:人胎盘,HBsAg和HIV抗体阴性

應用

用于细胞培养研究的

涂层材料胶原凝胶的形成

必须由最终用户确定最佳工作稀释度。

外觀

液体纯化蛋白。 在100μ L的0.5 M乙酸溶液中,pH 2.5。 无盐,无防腐剂。

儲存和穩定性

可在-20ºC保存长达12个月。 应避免反复冻/融循环。

分析報告

通过形成微纤维的能力证实了天然结构的保留。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Isolation and partial characterization of a new human collagen with an extended triple-helical structural domain.
Bentz, H, et al.
Proceedings of the National Academy of Sciences of the USA, 80, 3168-3172 (1983)
Characterization of a novel collagen chain in human placenta and its relation to AB collagen.
Sage, H and Bornstein, P
Biochemistry, 18, 3815-3822 (1979)
R W Glanville et al.
European journal of biochemistry, 95(2), 383-389 (1979-04-02)
Native type IV collagen was isolated from human placenta using pepsin solubilisation followed by fractional salt precipitation and chromatogarphic purification. The native preparation was characterised using amino acid analyses, disc gel electrophoresis, segment-long-spacing crystallites and immunological methods. Two component alpha

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