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一般說明
研究领域:细胞信号传导
过氧化氢酶是一种活性酶,存在于需氧生物中。它是一种铁血红素蛋白和四聚体。
过氧化氢酶是一种活性酶,存在于需氧生物中。它是一种铁血红素蛋白和四聚体。
應用
来自黑曲霉的过氧氢酶已用作:
- 艰难梭菌孢子外壳蛋白功能表征期间的阳性对照品。
- 过氧化氢酶溶液的组分,以使用酶制备GLOX缓冲液并保存秀丽隐杆线虫的胚胎,然后进行单分子荧光原位杂交(smFISH)研究
- 成像缓冲液的组分,以进行富血小板血浆的随机光学重构显微术(STORM)
- Todd Hewitt培养基加0.5%酵母提取物(THY)培养基的补充剂,以中和肺炎球菌中的H2O2
生化/生理作用
过氧化氢酶催化过氧化氢分解为水和氧气。四聚体的每个亚基都含有与铁结合的正铁血红素IX基团。该酶还可强烈结合NADP,NADP紧邻血红素基团。测得等电点为 6.5。催化活性的最适 pH 为 7.0。酶活性受3-氨基-1-H-1,2,4-三唑、氰化物、叠氮化物、羟胺、溴化氰、2-巯基乙醇、二硫苏糖醇、联茴香胺和硝酸盐的抑制。将过氧化氢酶与抗坏血酸盐或抗坏血酸盐/Cu2+进行孵育,会导致过氧化氢酶分子的降解。
过氧化氢酶参与催化过氧化氢(H2O2)降解为水和游离氢的过程。过氧化氢酶在商业上用于分解H2O2,因为其可在工艺术流程中作为抗菌剂使用。此外,在H2O2较低浓度下,它还能催化乙醇和酚类等电子供体的氧化。过氧化氢酶通过保护细胞免受较高水平的活性氧(ROS)显示其抗氧化活性。过氧化氢酶在慢性淋巴细胞白血病、胃癌、胶质瘤和黑色系瘤中的表达会增加。过氧化氢酶在急性髓性白血病、肺癌、胰腺癌、前列腺癌和皮肤癌(非黑色素瘤)中的水平会降低。因此,过氧化氢酶在癌症中表现出双重作用。
注意
不建议冷冻溶液。
單位定義
一单位酶可在pH7.0、25℃下每分钟分解1.0 μmol的H2O2,通过测量A240的下降速率可知H2O2浓度从10.3降至9.2 mM。
外觀
在 3.2 M (NH 4 ) 2 SO 4 溶液 (pH 6.0) 中的混悬液
分析報告
双缩脲法测蛋白
抑制劑
产品编号
说明
价格
抗體
相關產品
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
实验方案
This procedure may be used for all Catalase products.
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