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形狀
lyophilized powder
品質等級
比活性
100-300 units/mg protein
分子量
~175 kDa
成份
Protein, 65-85%
異物活動
Creatinase and urease ≤1%
Hexokinase and ATPase ≤0.1%
儲存溫度
2-8°C
應用
在临床分析中，该酶可与肌酸酶、肌氨酸脱氢酶或肌氨酸氧化酶以及甲醛脱氢酶结合用于酶法测定肌酐。
来自微生物的肌酐酶可通过与其他酶共固定进行肌酐测定而用于安培生物传感器的制备。
生化／生理作用
来自假单胞菌属的肌酐酶是一种每个亚基分子量为28.4 kDa的同六聚体酶。它是一种环状酰胺水解酶，可催化肌酸酐向肌酸的可逆性转化。每个单体在β-链的C末端和主要α-螺旋的N末端附近都含有一个双核锌中心。这些锌离子指示着活性位点的位置。
物理性質
等电点： 4.7
米氏常数：3.2 x 10‾2M（肌苷），5.7 x 10‾2M（肌酸）
结构：每摩尔的酶6个亚基（每个亚基结合一摩尔的锌）
抑制剂：Ag+、Hg++、N-溴代琥珀酰亚胺、EDTA
最适pH值： 6.5 − 7.5
最适温度： 70°C
pH稳定性： pH 7.5 − 9.0 (5°C，16小时)
热稳定性： 低于70°C（pH 7.5，30分钟）
米氏常数：3.2 x 10‾2M（肌苷），5.7 x 10‾2M（肌酸）
结构：每摩尔的酶6个亚基（每个亚基结合一摩尔的锌）
抑制剂：Ag+、Hg++、N-溴代琥珀酰亚胺、EDTA
最适pH值： 6.5 − 7.5
最适温度： 70°C
pH稳定性： pH 7.5 − 9.0 (5°C，16小时)
热稳定性： 低于70°C（pH 7.5，30分钟）
單位定義
在pH 8.0、25 °C条件下，一个单位将在每分钟内水解1.0 μmole的肌苷
外觀
含有蔗糖和BSA作为稳定剂的冻干粉末
分析報告
通过缩二脲法测定的蛋白质。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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实验方案
Protocol for the Assay Procedure for Creatininase
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