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品質等級
種類
Type VII
形狀
lyophilized powder
比活性
≥4 FALGPA units/mg solid
≥700 CDU/mg solid (CDU = collagen digestion units)
分子量
68-130 kDa
純化經由
chromatography
成份
Protein, ≥85% biuret
溶解度
TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C
異物活動
caseinase and clostripain ≤1 units/mg protein
儲存溫度
−20°C
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相关类别
應用
胶原酶已用于制备研究晚期糖基化终产物（AGE）的动脉组织。该酶还与其他蛋白酶一起用于人肿瘤、小鼠肾、人脑、肺上皮和许多其他组织的解聚。它在肝脏和肾脏灌注研究、胰腺消化和非实质肝细胞的分离中也是有效的。
生化／生理作用
从组织中有效释放细胞需要胶原酶和中性蛋白酶的共同作用。胶原酶被4克原子钙（Ca2+）/摩尔的酶活化。培养滤液至少含有7种分子量为68-130kDa的不同蛋白酶。最佳pH范围为6.3-8.8。该酶识别序列 -R-Pro-8-X-Gly-Pro-R-，其中X通常是中性氨基酸。
每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。
單位定義
一个胶原蛋白消化单元（CDU）从牛跟腱胶原蛋白中释放的肽链量和1.0 μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole 呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0 μmole 酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0 μmole BAEE。
外觀
含氯化钙的冻干粉
準備報告
储液制备：将0.05-0.1 mg胶原酶溶于1 mL含0.36 mM氯化钙的50 mM TES缓冲液(TESCA)中，pH 7.4(37 °C)。采用冷的缓冲液。
Please note the material will not dissolve completely and few sediments may be seen. The sediments do not affect the activity of the solution.
基底
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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实验方案
This procedure may be used for Collagenase products.
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