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Merck

C0412

Sigma-Aldrich

伴刀豆球蛋白A 来源于洋刀豆 (刀豆)

Type IV-S, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

别名:

刀豆素 A

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
23151817
NACRES:
NA.32

品質等級

無菌

γ-irradiated

種類

Type IV-S

產品線

BioReagent

形狀

lyophilized powder

技術

cell culture | mammalian: suitable

雜質

salt, essentially free

溶解度

PBS: 5 mg/mL, slightly hazy
H2O: soluble, slightly hazy

儲存溫度

−20°C

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應用

来自洋刀豆(刀豆)的伴刀豆球蛋白A已用于以下情形:
  • 诱导脾胚芽生殖
  • 从B6和lpr脾细胞生成ConA blast靶标
  • 刺激雌激素和安慰剂治疗小鼠的脾淋巴细胞

生化/生理作用

刀豆素 A 无血型特异性,但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca2+ 和 Mn2+ 离子存在才能显示活性。刀豆素 A 在 pH 6.5 或更低的条件下解离为二聚体。pH 在 5.8 至 7.0 之间时,刀豆素 A 以四聚体形式存在;pH 高于 7.0 时,形成更高的聚合体。根据聚合程度不同,刀豆素 A 可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。

分析報告

据报道,凝集活性以μg/ml表示,并且在Ca2+ 和Mn2+ 浓度为1 mg/ml、pH 6.8的磷酸缓冲盐溶液中进行连续稀释测定。该活性是在25°C下孵育1小时的2%人红细胞悬浮液可以凝集的最低浓度。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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