79390

磷酸酶(碱性) 来源于小牛肠粘膜

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

• 别名: Alkaline Phosphatase from calf intestinal mucosa
• CAS号: 9001-78-9
• EC 号: 3.1.3.1 (BRENDA, IUBMB)
• EC號碼: 232-631-4
• MDL號碼: MFCD00131849
• 分類程式碼代碼: 12352204
• eCl@ss: 42010105
• NACRES: NA.54

属性

• 生物源: bovine (calf) intestinal mucosa
• 形狀: solution (clear, colorless)
• 比活性: ~2500 U/mg protein (~10 mg/ml)
• 分子量: M_r ~140000
• 技術: enzyme immunoassay: suitable
• 雜質: 0.1 mM ZnCl₂; 1 mM MgCl₂; 3 M NaCl; 30 mM triethanolamine
• pH值: ~7.6
• 儲存溫度: 2-8°C

说明

應用

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

生化／生理作用

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

單位定義

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C

重構

The solution can be used without dialysis (for enzyme immuno-assay).

其他說明

Sales restrictions may apply

Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase

安全信息

• 象形圖: GHS08
• 訊號詞: Danger
• 危險聲明: H334
• 防範說明: P261 - P284 - P501
• 危險分類: Resp. Sens. 1
• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)