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Merck

C5896

Sigma-Aldrich

胆碱氧化酶 来源于产碱杆菌

lyophilized powder, ≥10 units/mg solid

别名:

胆碱:氧气 1-氧化还原酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder

品質等級

比活性

≥10 units/mg solid

分子量

~95 kDa

技術

cell based assay: suitable

適合性

suitable for assay of choline
suitable for molecular biology

儲存溫度

−20°C

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應用

来自产碱杆菌属的胆碱氧化酶已用于:
  • 在一项研究中研究用几丁质膜构建的胆碱生物传感器及其在测定胆碱酯酶抑制活性中的应用
  • 胆碱氧化酶与共价结合黄素的光反应的光谱研究。
  • 作为磷酸盐缓冲液 (PBS) 缓冲液的成分,用于测定脑组织匀浆中的胆碱乙酰转移酶 (ChAT) 活性
  • 通过 ATX 活性试验测定自分泌运动因子 (ATX) 产生的胆碱,通过 PLD 活性试验测定磷脂酶-D (PLD) 产生的胆碱

生化/生理作用

胆碱氧化酶可利用甜菜碱醛作为中间体而分子氧作为次要电子受体,催化胆碱至甘氨酸-甜菜碱的四电子氧化。该酶还可接受甜菜碱醛作为底物。这使得对将胆碱转化为醛中间体、& 甜菜碱醛转化为甘氨酸-甜菜碱的反应机制研究成为可能。该酶是一种分子量约为72,000 Da(凝胶过滤)或66,000 Da(SDS凝胶电泳)的黄酮蛋白。

物理性質

等电点:4.1 ± 0.1
米氏常数:2.84 x 10-3M (胆碱), 5.33 x 10¯3M (甜菜醛)
结构:一摩尔的FAD与一摩尔的酶共价结合
抑制剂:对氯苯甲酸,Cu++,Co++,Hg++,Ag+
最适pH:8.0 – 8.5
最适温度:40 – 45°C
pH稳定性: pH 7.0 – 9.0 (30°C, 2小时)
热稳定性: 低于37°C (pH 7.5, 10分钟)

單位定義

一单位的酶在37°C、pH 8.0条件下每分钟内可将1 μ摩尔的胆碱氧化成甜菜碱,并同时生成1 μ摩尔的H2O2。注:在通过胆碱氧化酶进行胆碱向甜菜碱的转化过程中,每μ摩尔的胆碱将产生2μ摩尔的H2O2

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Bo-Chuan Hsieh et al.
Journal of pharmaceutical and biomedical analysis, 45(4), 673-678 (2007-03-03)
An amperometric flow-injection choline biosensor was assembled utilizing natural chitinous membrane as the supporting material for biocatalyst immobilization, and the membrane was purified from Taiwanese soldier crab, Mictyris brevidactylus. The chitinous membrane (<50.0 microm in thickness) was covalently immobilized with
M Ohta-Fukuyama et al.
Journal of biochemistry, 88(1), 197-203 (1980-07-01)
Choline oxidase from Alcaligenes sp. catalyzed the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide. The values of Km for choline and betaine aldehyde were 0.87 and 6.2 mM, respectively.
Shihua Hou et al.
Biosensors & bioelectronics, 33(1), 44-49 (2012-01-11)
A novel acetylcholinesterase (AChE)/choline oxidase (ChOx) bienzyme amperometric acetylcholine biosensor based on gold nanoparticles (AuNPs) and multi-walled carbon nanotubes (MWCNTs) has been successfully developed by self-assembly process in combination of sol-gel technique. A thiolated aqueous silica sol containing MWCNTs and
Giovanni Gadda
Biochimica et biophysica acta, 1646(1-2), 112-118 (2003-03-15)
Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme is capable of accepting betaine-aldehyde as a substrate, allowing the investigation of the reaction mechanism for both
Shengyuan Deng et al.
Biosensors & bioelectronics, 26(11), 4552-4558 (2011-06-10)
A signal amplification system for electrochemiluminescence (ECL) of quantum dots (QDs) was developed by using electrochemically reduced graphene oxide (ERGO) to construct a nanobiosensing platform. Due to the structural defects of GO, the ECL emission of QDs coated on GO

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