推荐产品
生物源
mouse (Engelbreth-Holm-Swarm (EHS) mouse tumor)
無菌
sterile
化驗
≥90% (SDS-PAGE)
濃度
6 mg/mL
技術
cell culture | mammalian: suitable
溶解度
water: miscible
運輸包裝
dry ice
儲存溫度
−70°C
一般說明
3-D Culture Matrix™ Mouse Laminin I may be used as a gel on which to grow cells, or as a media additive alone or in concert with other basement membrane components, to study cellular growth and differentiation in three dimensions in vitro. To offer the most standardized Laminin I for use in 3-D cultures, a special process is employed to provide material at a standard concentration of 6 mg/ml (by absorbance and extinction coefficient).
外觀
Solution in Dulbecco′s Modified Eagle′s medium containing 10 μg/ml gentamycin sulfate and no phenol red.
其他說明
Functional Assay:
3-D Culture: Laminin I promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.
Cell Attachment: Tested for the ability to promote cell attachment and spreading of MG63 human osteosarcoma cells.
3-D Culture: Laminin I promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.
Cell Attachment: Tested for the ability to promote cell attachment and spreading of MG63 human osteosarcoma cells.
Sterility Testing:
No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test.No mycoplasma contamination detected by PCR.Endotoxin concentrations = 20 EU/ml by LAL assay.
No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test.No mycoplasma contamination detected by PCR.Endotoxin concentrations = 20 EU/ml by LAL assay.
Storage Conditions and Product Stability:
Product is stable for a minimum of 3 months from date of shipment when stored at -80 °C in a manual defrost freezer. For optimal stability, store at -80 °C in aliquots. Avoid repeated freeze/thaw cycles.
Product is stable for a minimum of 3 months from date of shipment when stored at -80 °C in a manual defrost freezer. For optimal stability, store at -80 °C in aliquots. Avoid repeated freeze/thaw cycles.
法律資訊
3-D Culture Matrix is a trademark of Trevigen, Inc.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Daniel Rüdiger et al.
Cell reports, 32(6), 108015-108015 (2020-08-14)
Endothelial tube formation on a reconstituted basement membrane (Matrigel) is a well-established in vitro model for studying the processes of angiogenesis and vasculogenesis. However, to date, the organizing principles that underlie the morphogenesis of this network and that shape the initial
Ruilin Tian et al.
Neuron, 104(2), 239-255 (2019-08-20)
CRISPR/Cas9-based functional genomics have transformed our ability to elucidate mammalian cell biology. However, most previous CRISPR-based screens were conducted in cancer cell lines rather than healthy, differentiated cells. Here, we describe a CRISPR interference (CRISPRi)-based platform for genetic screens in
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门