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11243217001

Roche

层粘蛋白

from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

别名:

层粘连蛋白 来源于 Engelbreth-Holm-Swarm 小鼠肉瘤基底膜

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About This Item

CAS号:
114956-81-9
MDL號碼:
MFCD00081739
分類程式碼代碼:
12352207

生物源

mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)

品質等級

100

無菌

sterile; sterile-filtered

化驗

≥90% (SDS-PAGE)

形狀

solution

分子量

900 kDa

包裝

pkg of 1 mg (2 ml)

製造商／商標名

Roche

技術

cell culture | mammalian: suitable

UniProt登錄號

Q61292

儲存溫度

−20°C

基因資訊

mouse ... Lamb2(16779)

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相关类别

一般說明

层粘连蛋白是从小鼠Engelbreth-Holm-Swarm (EHS)肉瘤的层粘连蛋白-巢蛋白复合物中分离得到的。层粘连蛋白是由α、β和γ 链亚基组成的异源三聚体。它是基底膜结构支架所需的糖蛋白。层粘连蛋白支持许多细胞类型的生长和分化，包括上皮细胞、内皮细胞、神经细胞、肌肉细胞和肝细胞。

生物活性：经测试可促进HT-1080细胞的贴壁。
特异性：对大多数哺乳动物细胞有活性。

應用

层粘连蛋白已经证明可促进多种细胞的附着和生长；可用于包被培养皿。它已被用于细胞贴壁测定。推荐以1-2μg/cm2用作细胞培养基质。最佳浓度取决于细胞类型以及应用或研究目标。
层粘连蛋白支持多种细胞类型的生长和分化，包括表皮、内皮、神经、肌肉和肝脏细胞。 建议将其按1-2 μg/cm2用作细胞培养的基质。 最佳的浓度的确取决于细胞类型以及应用或研究目标。

品質

纯度：≥90% (SDS-PAGE) (作为层粘连蛋白巢蛋白复合物，1:1)

序列

小鼠层粘连蛋白由三条多肽链组成（A：400 kD，B1：230 kD和B2：220 kD），通过二硫键连接，并且是糖基化的。

外觀

溶液，0.5 mg/ml层粘连蛋白，溶剂为0.15 M NaCl，2 mM EDTA，0.05 M Tris-HCl，pH 7.4，无菌
溶液，通过0.2 ?m孔径滤膜过滤

準備報告

工作浓度：2至5 μg/cm2
2至5 μg/cm2 ，用于包被细胞培养容器。
储存条件（工作溶液）：2至8°C
未稀释溶液可在2至8°C下稳定保存至少3个月。
溶液应在2至8°C或冰上小心解冻。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

nwg

閃點（°F）

does not flash

閃點（°C）

does not flash

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Julie V Harness et al.
PloS one, 6(1), e14499-e14499 (2011-01-21)
As human embryonic stem cell (hESC) lines can be derived via multiple means, it is important to determine particular characteristics of individual lines that may dictate the applications to which they are best suited. The objective of this work was
H Colognato et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 218(2), 213-234 (2000-06-08)
The laminins are a family of glycoproteins that provide an integral part of the structural scaffolding of basement membranes in almost every animal tissue. Each laminin is a heterotrimer assembled from alpha, beta, and gamma chain subunits, secreted and incorporated
Anindita Dutta et al.
International journal of clinical and experimental pathology, 3(3), 288-302 (2010-03-13)
The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility, gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study
Elias Theodorou et al.
Genes & development, 23(5), 575-588 (2009-03-10)
Neuronal differentiation is a complex process that involves a plethora of regulatory steps. To identify transcription factors that influence neuronal differentiation we developed a high throughput screen using embryonic stem (ES) cells. Seven-hundred human transcription factor clones were stably introduced
Plamena R Angelova et al.
Methods in molecular biology (Clifton, N.J.), 2202, 33-42 (2020-08-29)
Production of reactive oxygen species (ROS) in the mitochondria plays multiple roles in physiology, and excessive production of ROS leads to the development of various pathologies. ROS in the mitochondria are generated by various enzymes, mainly in the electron transporvt
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