SF006
ESGRO Complete 细胞消化液
The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.
别名:
Stem Cell Tested Accutase
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所有图片(1)
About This Item
分類程式碼代碼:
12352207
eCl@ss:
32160801
NACRES:
NA.75
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品質等級
形狀
liquid
製造商／商標名
Chemicon®
技術
cell culture | stem cell: suitable
輸入
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
運輸包裝
dry ice
一般說明
ESGRO Complete™ Accutase 是一种蛋白水解和胶原分解酶的细胞分离溶液，经确认可用于分离在无血清条件下用ESGRO Complete 克隆级培养基（目录号SF001-500）培养的小鼠胚胎干细胞。 Accutase 不含有哺乳动物或细菌来源产品。
應用
细胞分离：
1.将Accutase®在室温下解冻。
2.用无菌PBS洗涤孔板、烧瓶或珠子。
3.通过无菌程序添加Accutase至培养皿或烧瓶中（每75平方厘米表面积10 mL）。
4.经培养物放回37°C培养箱中，以分离细胞（5-10分钟）。
5.按常规操作进行细胞计数和传代。不需要额外的洗涤或酶抑制剂。
1.将Accutase®在室温下解冻。
2.用无菌PBS洗涤孔板、烧瓶或珠子。
3.通过无菌程序添加Accutase至培养皿或烧瓶中（每75平方厘米表面积10 mL）。
4.经培养物放回37°C培养箱中，以分离细胞（5-10分钟）。
5.按常规操作进行细胞计数和传代。不需要额外的洗涤或酶抑制剂。
外觀
冷冻无菌液体，即用型制剂。 每批都已经过无菌性（通过USP膜过滤方法）、酶活性（通过合成发色四肽测试）和细胞与组织培养塑料的分离性能测试。
1X accutase® 酶，溶于含0.5 mM EDTA•4Na和3 mg/L苯酚红的Dulbecco′s PBS中。
1X accutase® 酶，溶于含0.5 mM EDTA•4Na和3 mg/L苯酚红的Dulbecco′s PBS中。
儲存和穩定性
保存于-20°C可保持稳定。请参阅标签上的批次失效日期。 建议收货时保存于-20°C。 解冻后，Accutase® 可以在4°C下最多保存2个月。请勿在室温下存放。
法律資訊
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
cOmplete is a trademark of Roche
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Darren A Cusanovich et al.
Cell, 174(5), 1309-1324 (2018-08-07)
We applied a combinatorial indexing assay, sci-ATAC-seq, to profile genome-wide chromatin accessibility in ∼100,000 single cells from 13 adult mouse tissues. We identify 85 distinct patterns of chromatin accessibility, most of which can be assigned to cell types, and ∼400,000
Lina Dahl et al.
PloS one, 3(4), e2025-e2025 (2008-04-24)
The molecular mechanisms regulating the expansion of the hematopoietic system including hematopoietic stem cells (HSCs) in the fetal liver during embryonic development are largely unknown. The LIM-homeobox gene Lhx2 is a candidate regulator of fetal hematopoiesis since it is expressed
Thangaselvam Muthusamy et al.
Stem cell reports, 3(1), 169-184 (2014-07-30)
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