跳转至内容
Merck
所有图片(1)

Key Documents

View All Documentation

A6964

Sigma-Aldrich

Accutase® 溶液

sterile-filtered, suitable for cell culture

别名:

Cell detachment solution

登录查看公司和协议定价
所有图片(1)

About This Item

MDL號碼:
MFCD01632905
分類程式碼代碼:
12352204
NACRES:
NA.75

無菌

sterile-filtered

品質等級

200

形狀

liquid

濃度

400-600 units/mL

技術

cell culture | mammalian: suitable
single cell analysis: suitable

雜質

≤1 EU/mL endotoxin

pH值

6.8-7.8

運輸包裝

dry ice

儲存溫度

−20°C

相关类别

一般說明

Accutase®溶液（在不含Ca ++或Mg ++的杜氏磷酸缓冲盐溶液(DPBS)）包含用于细胞解离的蛋白水解酶和胶原蛋白水解酶。可用于从标准组织培养塑料器具和粘附涂层塑料器具中常规解离细胞。Accutase 是优于胰蛋白酶的细胞脱壁试剂，因为它有助于细胞从培养容器分离，损伤较少。因此，Accutase 常用于处理敏感的细胞或用于分析表面标志物。

應用

Accutase®溶液已用于将聚集簇中生长的非贴壁细胞（如脑肿瘤细胞、诱导性多能干细胞(IPSC)和胶质母细胞瘤细胞）解离或酶解成单个细胞。

适合制备测序用单细胞悬液。
证明可有效对原代成纤维细胞、内皮细胞、神经元、肿瘤细胞系和昆虫细胞进行解离。 在用于分析细胞表面标志物、病毒生长检测、流式细胞术以及生物反应器放大的细胞解离中表现尤为突出。不含有哺乳动物或细菌来源产品。

特點和優勢

由蛋白水解酶和胶原溶解酶的专有混合所组成的即用型液体用于将细胞从标准和带有粘附涂层的塑料器皿上进行解离。

公式變體

制备采用含0.5 mM EDTA•4Na以及3 mg/L酚红的Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl以及1.15 g/L Na2HPO4)。 可参考网站上的产品数据清单获取产品用途信息。

準備報告

该产品中含有酚红。该产品通过干冰进行运输所有在包装中会有显著的CO2累积。这些CO2可能会进入到溶液并略微降低pH，从而从淡粉色（约pH 7.8）转化为黄橘色（约pH 6.8）。该溶液如果呈现黄橘色（酸性）则仍然可以正常使用，或加入氢氧化钠对pH进行调节。

法律資訊

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

相關產品

产品编号
说明
价格

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

其他客户在看

Slide 1 of 1

1 of 1

Sigma-Aldrich

Sigma-Aldrich

D6546

杜氏改良 Eagle 培养基 - 高葡萄糖

Recent Zika virus isolates induce premature differentiation of neural progenitors in human brain organoids
Gabriel E, et al.
Cell Stem Cell, 20(3), 397-406 (2017)
Alvaro Cobo-Calvo et al.
Neurology(R) neuroimmunology & neuroinflammation, 6(2), e543-e543 (2019-02-26)
To describe clinical and radiologic features of cranial nerve (CN) involvement in patients with myelin oligodendrocyte glycoprotein antibodies (MOG-IgG) and to assess the potential underlying mechanism of CN involvement using a nonhuman primate (NHP) model. Epidemiologic, clinical, and radiologic features
Ruchi Bajpai et al.
Molecular reproduction and development, 75(5), 818-827 (2007-12-25)
Human embryonic stem cells (hESCs) hold great promise for cell-based therapies and drug screening applications. However, growing and processing large quantities of undifferentiated hESCs is a challenging task. Conventionally, hESCs are passaged as clusters, which can limit their growth efficiency
Frank-Peter Wachs et al.
Laboratory investigation; a journal of technical methods and pathology, 83(7), 949-962 (2003-07-16)
Neural stem cells (NSCs) from the adult central nervous system are currently being investigated for their potential use in autologous cell replacement strategies. High expansion rates of NSCs in culture are crucial for the generation of a sufficient amount of
DISC1 Regulates the Proliferation and Migration of Mouse Neural Stem/Progenitor Cells through Pax5, Sox2, Dll1 and Neurog2.
Qian Wu et al.
Frontiers in cellular neuroscience, 11, 261-261 (2017-09-14)
查看所有相关文献

商品

Human Mesenchymal Stem Cells and Growth/Differentiation Media

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and product use.

Human Mesenchymal Stem Cells and Growth/Differentiation Media

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and product use.

Neural Stem Cell FAQs

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

查看所有结果

实验方案

Human Mesenchymal Stem Cells and Growth/Differentiation Media

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and product use.

Alzheimer’s In A Dish™: 3D Neural Stem Cell Models of Alzheimer's Disease

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门