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SCR544

人STEMCCA组成型多顺反子（OKSM）慢病毒重编程试剂盒

Name: 人STEMCCA组成型多顺反子（OKSM）慢病毒重编程试剂盒
Brand: MM

The Human STEMCCA Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.

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About This Item

分類程式碼代碼:

12352207

eCl@ss:

32161000

NACRES:

NA.71

品質等級

100

製造商／商標名

STEMCCA

技術

cell culture | stem cell: suitable

輸入

sample type induced pluripotent stem cell(s)

運輸包裝

dry ice

一般說明

人STEMCCA组成型多顺反子（OKSM）慢病毒试剂盒包含高滴度多顺反子（OKSM）慢病毒和聚凝胺转染试剂，已经过验证可用于从人包皮成纤维细胞（HFF）生成人诱导多能干细胞。 STEMCCA载体由转录因子人Oct-4、Klf4、SOX-2和c-Myc（OKSM）组成，由自切割2A肽和IRES序列隔开（Sommer CA，2009）。使用单个多顺反子慢病毒载体可显著提高重编程效率并减少病毒整合的数量。

成分

1. 人STEMCCA组成型（OKSM）慢病毒：（部件号SCR544-1）两（2）瓶EF1α-hSTEMCCA （OKSM）慢病毒（部件号CS204502）。每瓶包含15 µL高滴度慢病毒。

2. 聚凝胺转染试剂：（部件号TR-1003-50UL）一（1）瓶包含50 µL 10 mg/mL储备液。

品質

测试以确认从p6人包皮成纤维细胞生成iPS细胞。尚未测试其他细胞类型，因此无法保证相似的结果。

儲存類別代碼

12 - Non Combustible Liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Chromosome microduplication in somatic cells decreases the genetic stability of human reprogrammed somatic cells and results in pluripotent stem cells.

Yang Yu et al.

Scientific reports, 5, 10114-10114 (2015-05-13)

Human pluripotent stem cells, including cloned embryonic and induced pluripotent stem cells, offer a limitless cellular source for regenerative medicine. However, their derivation efficiency is limited, and a large proportion of cells are arrested during reprogramming. In the current study

Generation of induced pluripotent stem cells using skin fibroblasts from patients with myocardial infarction under feeder-free conditions.

Jun-Quan Li et al.

Molecular medicine reports, 9(3), 837-842 (2014-01-09)

Myocardial infarction (MI) is an increasing medical problem; however, its pathogenesis has yet to be elucidated and more effective treatment strategies are required. Induced pluripotent stem cells (iPSCs) were recently successfully generated using human somatic cells transfected with four transcription

Amyotrophic lateral sclerosis patient iPSC-derived astrocytes impair autophagy via non-cell autonomous mechanisms.

Martin Madill et al.

Molecular brain, 10(1), 22-22 (2017-06-15)

Amyotrophic lateral sclerosis, a devastating neurodegenerative disease, is characterized by the progressive loss of motor neurons and the accumulation of misfolded protein aggregates. The latter suggests impaired proteostasis may be a key factor in disease pathogenesis, though the underlying mechanisms

商品

HumanKine® Thermostable bFGF, an engineered recombinant protein with enhanced biological functionality on human iPS and neural progenitor cells

Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmental pathways and help regulate mesoderm and ectoderm patterning in the early embryonic development.

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