MABF3137

Anti-MXRA8 Antibody, clone 9E9

• 别名: Dual Ig domain-containing cell adhesion molecule, Limitrin, Matrix remodeling-associated protein 8
• 分類程式碼代碼: 12352203
• NACRES: NA.46
• 無性繁殖: 9E9, monoclonal
• application: FACS; WB; inhibition assay
• 物種活性: human
• 技術: flow cytometry: suitable; inhibition assay: suitable; western blot: suitable

属性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 9E9, monoclonal
• 分子量: calculated mol wt 49.13 kDa
• 純化經由: using protein G
• 物種活性: human
• 物種活性（以同源性預測）: mouse
• 包裝: antibody small pack of 100
• 技術: flow cytometry: suitable; inhibition assay: suitable; western blot: suitable
• 同型: IgG1κ
• 表位序列: N-terminal
• Protein ID登錄號: NP_001269511
• UniProt登錄號: Q9BRK3
• 儲存溫度: -10 to -25°C
• 基因資訊: human ... MXRA8(54587)

说明

特異性

Clone 9E9 is a mouse monoclonal antibody that detects Matrix remodeling-associated protein 8 (MXRA8). It targets an epitope within 15 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 15 amino acids from the extracellular domain within the N-terminal region of human Matrix remodeling-associated protein 8 (MXRA8).

應用

Quality Control Testing

Evaluated by Flow Cytometry in THP-1 cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected MXRA8 in one million THP-1 cells.

Tested Applications

Inhibition Assay: A representative lot block MXRA8 activity and reduced TH17 cell trafficking across the blood brain barrier. (Charabati, M., et al. (2022). Sci Transl Med. 14(626): eabj0473).

Flow Cytometry Analysis: A representative lot detected MXRA8 in Flow Cytometry applications (Charabati, M., et al. (2022). Sci Transl Med.14(626): eabj0473).

Western Blotting Analysis: A representative lot detected MXRA8 in Western Blotting applications (Charabati, M., et al. (2022). Sci Transl Med. 14(626): eabj0473).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Matrix remodeling-associated protein 8 (UniProt: Q9BRK3; also known as Dual Ig domain-containing cell adhesion molecule, DICAM, MXRA8, Limitrin) is encoded by the MXRA8 gene (Gene ID: 54587) in human. MXRA8/DICAM is a single-pass type I, homodimeric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 20-341), a transmembrane domain (aa 342-362), and a cytoplasmic domain (aa 363-442). It is highly expressed at the areas of cell-cell contacts. It is also detected in the soluble fraction of the nucleus. Its high expression is observed in the brain where it localizes to glia limitans formed by the end feet of astrocytes surrounding capillaries, and beneath the pia mater. It is also expressed in intercalated disks in the heart and has been detected in pancreatic -cells. It modulates the activity of various signaling pathways via binding to integrin V:β3 via its RGD motif (aa 252-254). It plays a role in cartilage formation where it promotes proliferation and maturation of growth plate chondrocytes and stimulates the formation of primary cilia in chondrocytes. MXRA8 also plays a role in the maturation and maintenance of the blood-brain barrier. It is reported to function as a cell trafficking molecule that is preferentially expressed by T helper 17 (TH17)-polarized CD4+ T lymphocytes. Its expression on circulating CD4+ T cells is increased in patients with active relapsing-remitting multiple sclerosis (RRMS) and in progressive forms of multiple sclerosis. Expression of its ligands also increases on the blood-brain barrier endothelium upon inflammation and in multiple sclerosis lesions. Four isoforms of MXRA8 have been described that are produced by alternative splicing. (Ref.: Charabati, M., et al. (2022). Sci. Transl. Med. 14; eabj0473; Zhang, R., et al. (2018). Nature. 557(7706); 570-574).

外觀

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

重構

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable