推荐产品
生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
運輸包裝
wet ice
儲存溫度
−20°C
目標翻譯後修改
phosphorylation (pSer300)
一般說明
免疫亲和纯化的兔多克隆抗体。可识别出在Ser300处磷酸化的~44 kDa的PDH-E1α蛋白。
识别HEK293细胞中在Ser300磷酸化的~44 kDa PDH-E1α蛋白。
该PhosphoDetect抗-PDH-E1α pSer³⁰⁰）兔pAb经验证可用于免疫印迹法、免疫细胞化学检测PDH-E1α（pSer³⁰⁰）。
免疫原
人
合成磷酸肽，对应于人PDH-E1的Ser³⁰⁰磷酸化位点周围的氨基酸α
應用
免疫印迹（0.5μg/ml）
免疫细胞化学（1μg/ml）
包裝
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
外觀
在150 mM NaCl，100 mM Tris-甘氨酸，pH 7.4中。
重構
初次融化后，等分并冷冻（-20°C）。
分析報告
对照
HEK293细胞
HEK293细胞
阴性对照
用二氯乙酸盐处理的HEK293细胞
用二氯乙酸盐处理的HEK293细胞
其他說明
Rardin M.J., et. al. 2009.Anal.Biochem.2, 157.
Seifert, F., et al. 2007.Biochemistry 21, 6277.
Lee, J., et al. 2007.Mol.Cell Prot.4, 669.
Patel, M.S. and Korotchkina, L.G.2006 Biochem.Soc.Trans.34, 217.
Korotchkina, L.G., et al. 2001.J. Biol. Chem. 40, 37223.
Seifert, F., et al. 2007.Biochemistry 21, 6277.
Lee, J., et al. 2007.Mol.Cell Prot.4, 669.
Patel, M.S. and Korotchkina, L.G.2006 Biochem.Soc.Trans.34, 217.
Korotchkina, L.G., et al. 2001.J. Biol. Chem. 40, 37223.
由于高程度序列一致性，该抗体也预期与小鼠，大鼠，牛，斑马鱼和非洲爪蟾发生交叉反应。磷酸化PDH-E1α抗体应进行滴定以在单个系统中获得最佳结果。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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