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Millipore

BugBuster®预混液

别名:

细胞裂解预混液

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About This Item

分類程式碼代碼:
41106511
NACRES:
NA.32

形狀

liquid

品質等級

200

製造商／商標名

Novagen®

儲存條件

OK to freeze

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

BugBuster®预混液可用于高效的蛋白纯化及其它相关应用。它可取代高压破菌(French press)或超声波处理等机械方法。提供两种包装规格，分别包含足以从20 g或100 g细胞糊中提取蛋白的试剂。

應用

BugBuster®预混液的应用实例：
  • 在蛋白纯化实验中重悬和裂解细胞沉淀
  • 提取免疫印迹用蛋白
  • 作为细菌裂解缓冲液用于制备包涵体


    

生化／生理作用

BugBuster®蛋白提取试剂专门用于温和地破坏大肠杆菌细胞壁以释放可溶性蛋白。利用混合去垢剂的独特配方有效地穿破细胞壁但不会使可溶性蛋白变性。结合BugBuster试剂、rLysozyme溶液和Benzonase核酸酶，BugBuste预混液对蛋白的提取效率更高，获得的蛋白提取物下游处理更加方便。

特點和優勢

  • 为目标表达蛋白释放提供方便、快捷、高性价比的解决方案。
  • 该预混液无需稀释或进行额外的分离操作。

  • 革兰氏阳性和革兰氏阴性细菌的高效裂解
  • 最大限度回收活性可溶性蛋白
  • 能穿透细胞壁而不使可溶性蛋白变性
  • 用于制备高纯度包涵体
  • 与蛋白酶和磷酸酶抑制剂混合物兼容

警告

毒性：标准处理（A）

法律資訊

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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The β-barrel assembly machine (BAM) integrates β-barrel proteins into the outer membrane (OM) of Gram-negative bacteria. An essential BAM subunit (BamA) catalyzes integration by promoting the formation of a hybrid-barrel intermediate state between its own β-barrel domain and that of
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Protein thermodynamic stability is a fundamental physical characteristic that determines biological function. Furthermore, alteration of thermodynamic stability by macromolecular interactions or biochemical modifications is a powerful tool for assessing the relationship between protein structure, stability, and biological function. High-throughput approaches
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The collagen like domain Scl2 from Streptococcus pyogenes has been proposed as a potential biomedical material. It is non-cytotoxic and non-immunogenic and can be prepared in good yield in fermentation. The Scl2 collagen domain is about a quarter of the
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The bacterial ligase Sortase A (SA) and its mutated variants have become increasingly popular over the last years for post-translational protein modifications due to their unparalleled specificity and efficiency. The aim of this work was to study SA as a
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The living world is largely divided into autotrophs that convert CO2 into biomass and heterotrophs that consume organic compounds. In spite of widespread interest in renewable energy storage and more sustainable food production, the engineering of industrially relevant heterotrophic model

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