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Merck

PROTTWO

Sigma-Aldrich

ProteoPrep® Universal Extraction Kit

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

應用

本试剂盒含有最新的创新性去垢剂,同时使用了专门配制的试剂,以及为制备两种可直接供二维(2D)电泳使用的亚细胞组分而设计的优化实验方案。
  • 组分1:可溶性/细胞质蛋白
  • 组分2:膜蛋白
通过特殊的还原剂和烷化剂制备出的样本可以在2D凝胶中表现出更高的聚焦度,减少拖尾。ProteoPrep通用提取试剂盒由Proteome Systems和Sigma的科研人员合作设计。

特點和優勢

  • 创新的去垢剂制备方法 - 大幅提高了溶解度,可加载更多的蛋白质,提高了2D凝胶中低丰度蛋白的可见性。
  • 两种预混的增溶溶液 - 可以生成两个清洗区分的群落,易于进行2D分析。
  • 预测定的还原剂和烷化剂 - 试剂易于使用,可提供更高的IEF分辨率。
  • 预称重的干混物- 稳定且易于重新配制
  • 方便的包装 - 无废弃物。可按需选择用量。

法律資訊

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • C0356Protein Extraction Reagent Type 4 23 mL化学品安全说明书

  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5化学品安全说明书

  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56化学品安全说明书

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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W M Freeman et al.
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We present here a new approach that enabled the identification of a new protein from a bacterial strain with unknown genomic background using a combination of inverted PCR with degenerate primers derived from N-terminal protein sequences and high resolution peptide
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Abundant and hydrophilic nonmembrane proteins with isoelectric points below pH 8 are the predominant proteins identified in most proteomics projects. In yeast, however, low-abundance proteins make up 80% of the predicted proteome, approximately 50% have pl's above pH 8 and
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