688225

Caspase-1 Substrate VI, Fluorogenic

Fluorogenic caspase-1 substrate.

• 别名: Caspase-1 Substrate VI, Fluorogenic, Z-YVAD-AFC
• 经验公式（希尔记法）: C₃₃H₃₆F₃N₅O₁₀
• CAS号: 219137-85-6
• 分子量: 719.66
• MDL编号: MFCD01318881
• UNSPSC代码: 12352200
• NACRES: NA.77

属性

• 质量水平: 100
• 方案: 98% (HPLC)
• 表单: solid
• 制造商/商品名称: Calbiochem^®
• 储存条件: OK to freeze; desiccated; protect from light
• 颜色: white to off-white
• 溶解性: DMSO: 5 mg/mL
• 荧光: λ_ex ~400 nm; λ_em ~505 nm
• 运输: ambient
• 储存温度: −20°C
• SMILES字符串: FC(F)(F)c1c2c([o][c](c1)=O)cc(cc2)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)C)Cc3ccc(cc3)O)C(C)C)C)CC(=O)O
• InChI: 1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)/t16-,23-,24-,28-/m0/s1
• InChI key: GWKJISJKNKIJNP-DEQDFGQZSA-N

说明

一般描述

Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke′s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively.

Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

生化/生理作用

Cell permeable: no

Primary Target
Fluorogenic caspase-1 substrate

Product does not compete with ATP.

Reversible: no

警告

Toxicity: Standard Handling (A)

序列

Ac-Tyr-Val-Ala-Asp-AFC

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他说明

Jones, R.A., et al. 1998. Hepatology 27, 1632.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Walker, N.P., et al. 1994. Cell 78, 343.
Thornberry, N.A., et al. 1992. Nature 356, 768.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable