5.08741

DAPI Stain

• 别名: DAPI Stain, 4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O
• 经验公式（希尔记法）: C₁₆H₁₅N₅ · 2HCl
• CAS号: 28718-90-3
• 分子量: 350.25
• MDL號碼: MFCD00012681
• 分類程式碼代碼: 41116130
• NACRES: NA.21

属性

• 化驗: ≥97% (HPLC)
• 品質等級: 200
• 形狀: liquid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• 螢光: λ_ex ~359 nm; λ_em ~461 nm
• 儲存溫度: −20°C
• SMILES 字串: Cl.Cl.[nH]1c2c(cc1c3ccc(cc3)\C(=N\[H])\N)ccc(c2)\C(=N\[H])\N
• InChI: 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• InChI 密鑰: FPNZBYLXNYPRLR-UHFFFAOYSA-N

说明

一般說明

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.

Cells are typically stained at 1 µg/ml.

應用

• Determination of Ploidy Levels and Nuclear DNA Content in Cryptococcus neoformans by Flow Cytometry: Drawbacks with Variability: This research utilizes DAPI for quantifying nuclear DNA content and determining ploidy levels in Cryptococcus neoformans, providing critical insights into fungal genetics and potential challenges in reproducibility (Chang et al., 2024).


• Near-Infrared Light-Excited Quinolinium-Carbazole Small Molecule as Two-Photon Fluorescence Nucleic Acid Probe: Highlights the development of a novel two-photon fluorescence probe for nucleic acid imaging, employing DAPI as a reference for comparing fluorescence characteristics, enhancing molecular imaging techniques (Sun et al., 2024).


• A FACS-based Novel Isolation Technique Identifies Heterogeneous CTCs in Oral Squamous Cell Carcinoma: Applies DAPI for the enumeration and characterization of circulating tumor cells (CTCs), showcasing its importance in cancer diagnostics and the potential for personalized medicine approaches (Chauhan et al., 2024).

生化／生理作用

Cell permeable: yes

Primary Target
DNA

Reversible: yes

包裝

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

外觀

A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.

重構

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

其他說明

Mathieu, C., et al. 1995. Exp. Cell Res.216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C.105, 251.
Hotz, M.A., et al. 1994. Cytometry15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods26, 249.
Miller, T., et al. 1993. Biotechniques15, 1042.

Gray, J.W., et al. 1982. Science238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods38, 315.

Russels, W.C., et al. 1975. Nature253, 461.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable